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The cancellation factor (CF) is a model for the ratio between gravity wave perturbations in the nightglow intensity to those in the ambient temperature. The CF model allows us to estimate the momentum and energy flux of gravity waves seen in nightglow images, as well as the divergence of these fluxes due to waves propagating through the mesosphere and lower thermosphere region, where the nightglow and the Na layers are located. This study uses a set of wind/temperature Na lidar data and zenith nightglow image observations of the OH and O(1S) emissions to test and validate the CF model from the experimental perspective. The dataset analyzed was obtained during campaigns carried out at the Andes Lidar Observatory (ALO), Chile, in 2015, 2016, and 2017. The modeled CF was compared with observed CF values calculated using the ratio of wave amplitude in nightglow images to that seen in lidar temperatures for vertically propagating waves. We show that, in general, the modeled CF underestimates the observed CF results. However, the O(1S) emission line has better agreement with respect to the modeled value due to its supposedly simpler nightglow photochemistry. In contrast, the observed CF for the OH emission deviates by a factor of two from the modeled CF asymptotic value.